List of publications

Experimental Biomolecular Physics

2014

• Hans Blom, Jerker Widengren. STED microscopy -- towards broadened use and scope of applications. Current Opinion in Chemical Biology, Volume 20, June 2014, Pages 127-133.
• Heike Hevekerl, Jens Wigenius, Gustav Persson, Olle Inganäs, Jerker Widengren. Dark states in ionic oligothiophene bioprobes - evidence from fluorescence correlation spectroscopy and dynamic light scattering. The Journal of Physical Chemistry B. 2014 118 (22), pp 5924-5933. DOI:10.1021/jp501324e.
• Kronqvist N, Otikovs M, Chmyrov V, Chen G, Andersson M, Nordling K, Landreh M, Sarr M, Jörnvall H, Wennmalm S, Widengren J, Meng Q, Rising A, Otzen D, Knight S, Jaudzems K, Johansson J. Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation. Nature Commun 5, 3254, 2014.
• Bergstrand J, Rönnlund D, Widengren J, Wennmalm S. Scanning Inverse Fluorescence Correlation Spectroscopy. Optics Express Vol. 22, No. 11, 13073-13090, 2014.
• Daniel Rönnlund , Lei Xu , Anna Perols , Annica KB Gad , Amelie Eriksson, Karlström , Gert Auer , and Jerker Widengren. Multicolor Fluorescence Nanoscopy by Photobleaching: Concept, Verification, and Its Application To Resolve Selective Storage of Proteins in Platelets. ACS Nano 2014, 8 (5), pp 4358-4365.
• Jonas Mücksch, Thiemo Spielmann, Evangelos Sisamakis, Jerker Widengren. Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse trains. Journal of Biophotonics 04/2014 (in press) DOI:10.1002/jbio.201400015.
• Xingqi Chen, Chengxi Shi, Samer Yammine, Anita Göndör, Daniel Rönnlund, Alejandro Fernandez-Woodbridge, Noriyuki Sumida, Jerker Widengren, and Rolf Ohlsson. Chromatin in situ proximity (ChrISP): Single-cell analysis of chromatin proximities at a high resolution. BioTechniques, Vol. 56, No. 3, March 2014, pp. 117-124.
• Annica K B Gad, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström. Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy. Methods in molecular biology (Clifton, N.J.) 01/2014; 1120:339-57. DOI:10.1007/978-1-62703-791-4_22.
• Ramnath Lakshmanan, Margarita Sanchez-Dominguez, Jose A. Matutes-Aquino, Stefan Wennmalm, and Gunaratna Kuttuva Rajarao. Removal of Total Organic Carbon from Sewage Wastewater Using Poly(ethylenimine)-Functionalized Magnetic Nanoparticles. Langmuir 2014, 30 (4), pp 1036-1044, DOI:10.1021/la404076n.
• Thiemo Spielmann, Lei Xu, Annica K.B. Gad, Sofia Johansson, Jerker Widengren. Transient state microscopy probes patterns ofaltered oxygen consumption in cancer cells. FEBS J Volume 281, Issue 5, pages 1317-1332, 2014; DOI:10.1111/febs.12709.
• Li-Sophie Z. Rathje, Niklas Nordgren, Torbjörn Pettersson, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström, and Annica K. B. Gad. Oncogenes induce a vimentin filament collapse mediated by HDAC6 that is linked to cell stiffness. PNAS vol. 111 no. 4 1515-1520 2014.

2013

• Hans Blom, Daniel Rönnlund, Lena Scott, Linda Westin, Jerker Widengren, Anita Aperia, Hjalmar Brismar. Spatial Distribution of DARPP-32 in Dendritic Spines. PloS ONE 8(9) 2013: e75155. doi:10.1371/journal.pone.0075155.
• Daniel Rönnlund, Annica K. B. Gad, Hans Blom, Pontus Aspenström, Jerker Widengren. Spatial Organization of Proteins in Metastasizing Cells. Cytometry Part A 2013, Vol. 89, Issue 9, pages 855-865.
• Jerker Widengren. Studying Ion Exchange in Solution and at Biological Membranes by FCS. Methods in Enzymology, Volume 519, 231-252.
• Paul J. Rothwell, William J. Allen, Evangelos Sisamakis, Stanislav Kalinin, Suren Felekyan, Jerker Widengren, Gabriel Waksman and Claus A. M. Seidel. dNTP-dependent Conformational Transitions in the Fingers Subdomain of Klentaq1 DNA Polymerase INSIGHTS INTO THE ROLE OF THE "NUCLEOTIDE-BINDING" STATE. The Journal of Biological Chemistry, 288 (19), 13575-13591., doi: 10.1074/jbc.M112.432690.

2012

• Stefan Wennmalm and Jerker Widengren. Interferometry and Fluorescence Detection for Simultaneous Analysis of Labeled and Unlabeled Nanoparticles in Solution. J. Am. Chem. Soc., 2012, 134 (48), pp 19516-19519. DOI: 10.1021/ja308213q.
• Daniel Rönnlund, Yang Yang, Hans Blom, Gert Auer, Jerker Widengren. Fluorescence Nanoscopy of Platelets Resolves Platelet-State Specific Storage, Release and Uptake of Proteins, Opening for Future Diagnostic Applications. Adv Healthcare Mat 2012, online: 7 Sep, DOI: 10.1002/adhm.201200172.
• Michele Cristovao, Evangelos Sisamakis, Manju M. Hingorani, Andreas D. Marx, Caroline P. Jung, Paul J. Rothwel,, Claus A. M. Seidel, and Peter Friedhoff. Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA. Nucl. Acids Res. (2012), 40 (12):5448-5464, doi:10.1093/nar/gks138.
• Annica K. B. Gad, Daniel Rönnlund, Alexander Spaar, Andrii A. Savchenko, Gabor Petranyi, Hans Blom, Laszlo Szekely, Jerker Widengren and Pontus Aspenström. Rho GTPases link cellular contractile force to the density and distribution of nanoscale adhesions. FASEB J 2012, vol. 26, no. 6 2374-2382.
• Peter Mellroth, Robert Daniels, Alice Eberhardt, Daniel Rönnlund, Hans Blom, Jerker Widengren, Staffan Normark and Birgitta Henriques-Normark. LytA, the major autolysin of Streptococcus pneumoniae, requires access to the nascent peptidoglycan. J Biol Chem 2012 Vol. 287, 14:11018-11029.
• Karl Skoog, Bill Söderström, Jerker Widengren, Gunnar von Heijne and Daniel O. Daley. Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli. J. Bacteriol. February 2012 vol. 194 no. 3:584-586.
• Tor Sandén, Romain Wyss, Christian Santschi, Ghérici Hassaïne, Cédric Deluz, Olivier J.F. Martin, Stefan Wennmalm, and Horst Vogel. A Zeptoliter Volume Meter for Analysis of Single Protein Molecules. Nano Lett 2012, 12(1), 370-375.
• Hans Blom, Daniel Rönnlund, Lena Scott, Zuzana Spicarova, Ville Rantanen, Jerker Widengren, Anita Aperia and Hjalmar Brismar. Nearest neighbor analysis of dopamine D1 receptors and Na+-K+-ATPases in dendritic spines dissected by STED microscopy. Microscopy Research and Technique 2012 Vol. 75, Issue 2, 220-228.

2011

• Heike Hevekerl, Thiemo Spielmann, Andriy Chmyrov, and Jerker Widengren. Förster Resonance Energy Transfer beyond 10 nm: Exploiting the Triplet State Kinetics of Organic Fluorophores. J Phys Chem B, 2011, 115 (45), pp 13360-13370.
• Johan Strömqvist, Sofia Johansson, Lei Xu, Yu Ohsugi, Katja Andersson, Hideki Muto, Masataka Kinjo, Petter Höglund and Jerker Widengren. A Modified FCCS Procedure Applied to Ly49A-MHC Class I cis-Interaction Studies in Cell Membranes. Biophysical Journal 5, 1257-1269, 2011.
• Jens Wigenius, Gustav Persson, Jerker Widengren, Olle Inganäs. Interactions Between a Luminescent Conjugated Oligoelectrolyte and Insulin. Macromolecular Bioscience 11, 1120-1127, 2011.
• Stefan Wennmalm, Jerker Widengren. Inverse-fluorescence correlation spectroscopy: more information and less labeling. Frontiers in Bioscience S3, 385-392, January 1, 2011.
• Hans Blom, Daniel Ronnlund, Lena Scott, Zuzana Spicarova, Jerker Widengren, Alexander Bondar, Anita Aperia and Hjalmar Brismar. Spatial distribution of Na+,K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy. BMC Neuroscience 2011, 12:16/1471-2202-12-16.

2010

• Johan Strömqvist, Andriy Chmyrov, Sofia Johansson, August Andersson, Lena Mäler and Jerker Widengren. Quenching of Triplet State Fluorophores for Studying Diffusion-Mediated Reactions in Lipid Membranes. Biophys J. 2010, Volume 99, Issue 11, 3821-3830.
• Andriy Chmyrov, Tor Sandén and Jerker Widengren. Recovery of Photoinduced Reversible Dark States Utilized for Molecular Diffusion Measurements. Anal. Chem., 2010, 82 (24), pp 9998-10005.
• Andriy Chmyrov, Tor Sandén and Jerker Widengren. Iodide as a Fluorescence Quencher and Promoter--Mechanisms and Possible Implications. J. Phys. Chem. B, 2010, 114 (34), pp 11282-11291.
• Evangelos Sisamakis, Alessandro Valeri, Stanislav Kalinin, Paul J. Rothwell and Claus A.M. Seidel. Chapter 18 - Accurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection. In: Methods in Enzymology Volume 475, 2010, Pages 455-514.
• Stefan Wennmalm and Jerker Widengren. Inverse-Fluorescence Cross-Correlation Spectroscopy. Anal. Chem. 2010, 82, 5646-5651.
• Stanislav Kalinin, Evangelos Sisamakis, Steven W. Magennis, Suren Felekyan and Claus A. M. Seidel. On the Origin of Broadening of Single-Molecule FRET Efficiency Distributions beyond Shot Noise Limits. J. Phys. Chem. B, 2010, 114 (18), 6197-6206.
• Widengren J. Fluorescence-based transient state monitoring for biomolecular spectroscopy and imaging. Journal of the Royal Society Interface August 6, 2010 7:1135-1144.
• Tor Sandén, Lina Salomonssona, Peter Brzezinski, and Jerker Widengren. Surface-coupled proton exchange of a membrane-bound proton acceptor. Proc. Natl. Acad. Sci. USA 2010 vol. 107(9), 4129-4134.
• Johan Strömqvist, Karl Skoog, Daniel O. Daley, Jerker Widengren and Gunnar von Heijne. Estimating Z-ring radius and contraction in dividing Escherichia coli. Molecular Microbiology 2010, 76(1), 151-158.
• Thiemo Spielmann, Hans Blom, Matthias Geissbuehler, Theo Lasser and Jerker Widengren. Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy. J. Phys. Chem. B 2010, 114 (11), 4035-4046.
• Hans Blom, Kai Hassler, Andriy Chmyrov and Jerker Widengren. Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy. Int. J. Mol. Sci. 2010, 11(2), 386-406.

2009

• Widengren J. Fluorescence flicker as a read-out in FCS - principles, applications and further developments. in Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Verlag 2009, Eds. Gräslund, Rigler, Widengren.
• Hongzhen Lin, Yuxi Tian, Karolina Zapadka, Gustav Persson, Daniel Thomsson, Oleg Mirzov, Per-Olof Larsson, Jerker Widengren, and Ivan G. Scheblykin. Fate of Excitations in Conjugated Polymers: Single-Molecule Spectroscopy Reveals Nonemissive "Dark" Regions in MEH-PPV Individual Chains. Nano letters, 2009, 9 (12), pp 4456-4461.
• Stefan Wennmalm, Per Thyberg, Lei Xu and Jerker Widengren. Inverse-Fluorescence Correlation Spectroscopy. Anal. Chem., 2009, 81 (22), pp 9209-9215.
• Hans Blom and Gunnar Björk. Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy. Applied Optics, Vol. 48, Issue 31, pp. 6050-6058.
• Gustav Persson, Tor Sandén, AnnSofi Sandberg and Jerker Widengren. Fluorescence cross-correlation spectroscopy of a pH-sensitive ratiometric dye for molecular proton exchange studies. Phys Chem Chem Phys, 2009, 11, 4410-4418.
• Gustav Persson, Per Thyberg, Tor Sandén and Jerker Widengren. Modulation Filtering Enables Removal of Spikes in Fluorescence Correlation Spectroscopy Measurements without Affecting the Temporal Information. J Phys Chem B, 2009, 113 (25), 8752-7.
• Hans Blom, Andriy Chmyrov, Kai Hassler, Lloyd M. Davis and Jerker Widengren. Triplet-State Investigations of Fluorescent Dyes at Dielectric Interfaces Using Total Internal Reflection Fluorescence Correlation Spectroscopy. J. Phys. Chem. A, 2009, 113 (19), 5554-66.
• Gustav Persson, Tor Sandén, Per Thyberg, and Jerker Widengren. Modulated or alternating excitation in fluorescence correlation spectroscopy. Proc. SPIE, Vol. 7185, 718509 (2009); doi:10.1117/12.808912.
• Tor Sandén, Gustav Persson, Jerker Widengren. Transient state microscopy: a new tool for biomolecular imaging (Proceedings Paper). Proceedings Vol. 7183. Multiphoton Microscopy in the Biomedical Sciences IX, Ammasi Periasamy; Peter T. C. So, Editors, 71832R.
• Öjemyr L, Sandén T, Widengren J, and Brzezinski P. Lateral Proton Transfer between the Membrane and a Membrane Protein. Biochemistry, 2009, 48 (10), 2173-2179.

2008

• Chmyrov A, Arden-Jacob J, Zilles A, Drexhage KH and Widengren J. Characterization of new fluorescent labels for ultra-high resolution microscopy. Photochem. Photobiol. Sci., 2008, 7, 1378-1385.
• Sandén T, Persson G, and Widengren J. Transient State Imaging for Microenvironmental Monitoring by Laser Scanning Microscopy. Anal Chem, 2008, 80 (24), 9589-9596.
• Persson G, Thyberg P, Widengren J. Modulated Fluorescence Correlation Spectroscopy with Complete Time Range Information. Biophysical Journal vol. 94, 977-85 2008.
• Sonesson A, Blom H, Hassler K, Elofsson U M, Callisen T H, Widengren J, Brismar H. Protein-surfactant interactions at hydrophobic interfaces studied with total internal reflection fluorescence correlation spectroscopy (TIR-FCS). Journal of Colloid and Interfaces Science 317, 449-57, 2008.

2007

• Sandén T, Persson G, Thyberg P, Blom H, Widengren J. Monitoring kinetics of highly environment-sensitive states of fluorescent molecules by modulated excitation and time-averaged fluorescence intensity recording. Anal. Chem. 79(9), 3330-3341, 2007.
• Widengren J, Chmyrov A, Eggeling C, Löfdahl PÅ, Seidel CAM. Strategies to improve photostability in ultrasensitive fluorescence spectroscopy. J. Phys. Chem. A 111(3), 429-440, 2007.
• Hassler K, Rigler P, Blom H, Rigler R, Widengren J, Lasser T. Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy. Optics Express, 15(9), 5366-5375, 2007.

2006

• Widengren J, Kudryavtsev V, Gerken M, Berger S, Seidel CAM. Single molecule detection and identification of multiple species by multiparameter fluorescence detection. Analytical Chemistry 78(6); 2039-2050, 2006.
• Eggeling C, Widengren J, Brand L, Schaffer J, Felekyan S, Seidel CAM. "Analysis of Acceptor Photobleaching in Single-Molecule Förster Resonance Energy Transfer Measurements". J. Phys. Chem A 110(9); 2979-2995, 2006
• Leutenegger M, Blom H, Widengren J, Eggeling C, Gösch M, Leitgeb RA, Lasser T. Dual-color total internal reflection fluorescence cross-correlation spectroscopy. J Biomed Optics, 11(4), 2006.
• Brändén M, Sandén T, Brzezinski P, Widengren J. Localised proton microcircuits at the biological membrane-water interface. Proc. Natl. Acad. Sci. U.S.A. 103(52), 19766-19770, 2006.
• Blom H, Kastrup L, Eggeling C. Fluorescence Fluctuation Spectroscopy in Reduced Detection Volumes. Current Pharmaceutical Biotechnology. 7(1), 51-66, 2006.

2005

• Widengren J, Thyberg P. FCS cell surface measurements - photophysical limitations and consequences on molecular ensembles with heterogenic mobilities. Cytometry part A, Vol. 68, 2: 101-112, 2005.

2004

• Fridberger A, Widengren J, Boutet de Monvel J. Measuring hearing organ vibration patterns with confocal microscopy and optical flow. Biophys. J. 86 (1): 535-543, 2004.
• Pramanik A, Widengren J. Fluorescence Correlation Spectroscopy (FCS). in Encyclopedia of Molecular Cell Biology and Molecular Medicine, Wiley-VCH Verlag 2004, p. 461-500, ed. Meyers

2003

• Margittai M, Widengren J, Schweinberger E, Schroder GF, Felekyan S, Haustein E, Konig M, Fasshauer D, Grubmuller H, Jahn R, Seidel CAM. Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1. Proc. Natl. Acad. Sci. U.S.A. 100 (26): 15516-15521, 2003.