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List of publications

Experimental Biomolecular Physics

List maintained by diva

List maintained by us

• 2023
• 2022
• 2021
• 2020
• 2019
• 2018
• 2017
• 2016
• 2015
• 2014
• 2013
• 2012
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• Doctoral Theses

2023

• Elin Sandberg, Joachim Piguet, Uliana Kostiv, Glib Baryshnikov, Haichun Liu, and Jerker Widengren.
  Photoisomerization of Heptamethine Cyanine Dyes Results in Red-Emissive Species: Implications for Near-IR, Single-Molecule, and Super-Resolution Fluorescence Spectroscopy and Imaging.
  J. Phys. Chem. B 2023, 127, 14, 3208-3222. https://doi.org/10.1021/acs.jpcb.2c08016.


• Lucía Labrador-Páez, Jouko Kankare, Iko Hyppänen, Tero Soukka, Elina Andresen, Ute Resch-Genger, Jerker Widengren, and Haichun Liu.
  Frequency-Domain Method for Characterization of Upconversion Luminescence Kinetics.
  J. Phys. Chem. Lett. 2023, 14, 14, 3436-3444. https://doi.org/10.1021/acs.jpclett.3c00269.


• Elin Sandberg, Joachim Piguet, Haichun Liu and Jerker Widengren.
  Combined Fluorescence Fluctuation and Spectrofluorometric Measurements Reveal a Red-Shifted, Near-IR Emissive Photo-Isomerized Form of Cyanine 5.
  Int. J. Mol. Sci. 2023, 24, 1990. https://doi.org/10.3390/ijms24031990.


• Akira Kitamura, Johan Tornmalm, Bariş Demirbay, Joachim Piguet, Masataka Kinjo and Jerker Widengren.
  Trans-cis isomerization kinetics of cyanine dyes reports on the folding states of exogeneous RNA G-quadruplexes in live cells.
  Nucleic Acids Research, 2023 https://doi.org/10.1093/nar/gkac1255.


• Fuhua Huang, Niusha Bagheri, Li Wang, Hans Ågren, Jinglai Zhang, Jerker Widengren, Haichun Liu.
  Low-lying excited state energy trap induced by cross-relaxation - The main origin of concentration quenching in lanthanide upconversion nanoparticles.
  Journal of Alloys and Compounds 936 (2023) 168149.


• Fuhua Huang, Lucía Labrador-Páez, Hans Ågren, Li Wang, Jinglai Zhang, Rui Pu, Qiuqiang Zhan, Jerker Widengren, Haichun Liu.
  Transient energy trapping as a size-conserving surface passivation strategy for producing bright ultrasmall upconversion nanoprobes.
  Nano Energy 105 (2023) 108015

2022

• Lucía Labrador-Páez, Uliana Kostiv, Qingyun Liu, Yuanyuan Li, Hans Ågren, Jerker Widengren, and Haichun Liu.
  Excitation Pulse Duration Response of Upconversion Nanoparticles and Its Applications.
  J. Phys. Chem. Lett. 2022, 13, 11208-11215


• Fuhua Huang, Niusha Bagheri, Li Wang, Hans Ågren, Jinglai Zhang, Jerker Widengren, Haichun Liu.
  Low-lying excited state energy trap induced by cross-relaxation – The main origin of concentration quenching in lanthanide upconversion nanoparticles.
  Journal of Alloys and Compounds 936 (2023) 168149.


• Jan Bergstrand, Xinyan Miao, Chinmaya Venugopal Srambickal, Gert Auer and Jerker Widengren.
  Fast, streamlined fluorescence nanoscopy resolves rearrangements of SNARE and cargo proteins in platelets co-incubated with cancer cells.
  Journal of Nanobiotechnology (2022) 20:292.


• Yusen Liang, Zhimin Zhu, Shuqian Qiao, Xin Guo, Rui Pu, Huan Tang, Haichun Liu, Hao Dong, Tingting Peng, Ling-Dong Sun, Jerker Widengren and Qiuqiang Zhan.
  Migrating photon avalanche in different emitters at the nanoscale enables 46th-order optical nonlinearity.
  Nature Nanotechnology Vol 17, May 2022, 524-530.


• Lucía Labrador-Páez, Uliana Kostiv, Jerker Widengren, Haichun Liu.
  Water: An Influential Agent for Lanthanide-Doped Luminescent Nanoparticles in Nanomedicine.
  Adv. Optical Mater.2022, 2200513. DOI: 10.1002/adom.202200513.


• Xin Guo, Rui Pu, Zhimin Zhu, Shuqian Qiao, Yusen Liang, Bingru Huang, Haichun Liu, Lucía Labrador-Páez, Uliana Kostiv, Pu Zhao, Qiusheng Wu, Jerker Widengren & Qiuqiang Zhan.
  Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes.
  Nature Communications volume 13, Article number: 2843 (2022) https://doi.org/10.1038/s41467-022-30114-z.


• Sejoo Jeong, Jerker Widengren and Jong-Chan Lee.
  Fluorescent Probes for STED Optical Nanoscopy.
  Nanomaterials 2022, 12(1), 21; https://doi.org/10.3390/nano12010021.


• Maryam Sanaee, Elin Sandberg, K. Göran Ronquist, Jane M. Morrell, Jerker Widengren, Katia Gallo.
  Coincident Fluorescence-Burst Analysis of the Loading Yields of Exosome-Mimetic Nanovesicles with Fluorescently-Labeled Cargo Molecules.
  Small 2022, 2106241.


• Caroline A. Evans, Hyejeong Rosemary Kim, Sarah C. Macfarlane, Poppy I. A. Nowicki, Carsten Baltes, Lei Xu, Jerker Widengren, Franziska Lautenschläger, Bernard M. Corfe and Annica K. B. Gad.
  Metastasising Fibroblasts Show an HDAC6-Dependent Increase in Migration Speed and Loss of Directionality Linked to Major Changes in the Vimentin Interactome.
  Int. J. Mol. Sci. 2022, 23, 1961.

2021

• Tingting Peng, Rui Pu, Baoju Wang, Zhimin Zhu, Kai Liu, Fan Wang, Wei Wei, Haichun Liu and Qiuqiang Zhan.
  The Spectroscopic Properties and Microscopic Imaging of Thulium-Doped Upconversion Nanoparticles Excited at Different NIR-II Light.
  Biosensors 2021, 11, 148.


• Christoph Dumke, Timo Gemoll, Martina Oberländer, Sandra Freitag-Wolf, Christoph Thorns, Axel Glaessgen, Rinse Klooster, Silvère M. van der Maarel, Jerker Widengren, Christian Doehn, Gert Auer & Jens K. Habermann.
  SATB1, genomic instability and Gleason grading constitute a novel risk score for prostate cancer.
  Scientific Reports (2021) 11:244.


• Mahebali Tabusi, Sigrun Thorsdottir, Maria Lysandrou, Ana Rita Narciso, Melania Minoia, Chinmaya Venugopal Srambickal, Jerker Widengren, Birgitta Henriques-Normark, Federico Iovino.
  Neuronal death in pneumococcal meningitis is triggered by pneumolysin and RrgA interactions with β-actin.
  PLOS Pathogens March 24, 2021.


• Chinmaya Venugopal Srambickal, Jan Bergstrand and Jerker Widengren.
  Cumulative effects of photobleaching in volumetric STED imaging - artefacts and possible benefits.
  Methods and Applications in Fluorescence 9 (2021) 015003.

2020

• Vicky Sender, Karina Hentrich, Anuj Pathak, Alicia Tan Qian Ler, Bethel Tesfai Embaie, Susanna L. Lundström, Massimiliano Gaetani, Jan Bergstrand, Rei Nakamoto, Lok-To Sham, Jerker Widengren, Staffan Normark, and Birgitta Henriques-Normark.
  Capillary leakage provides nutrients and antioxidants for rapid pneumococcal proliferation in influenza-infected lower airways.
  PNAS December 8, 2020 117 (49) 31386-31397. https://www.pnas.org/content/117/49/31386.


• Yanan Ji, Wen Xu, Nan Ding, Haitao Yang, Hongwei Song, Qingyun Liu, Hans Ågren, Jerker Widengren & Haichun Liu.
  Huge upconversion luminescence enhancement by a cascade optical field modulation strategy facilitating selective multispectral narrow-band near-infrared photodetection.
  Light Sci Appl 9, 184 (2020). https://doi.org/10.1038/s41377-020-00418-0.


• Zhaoyang Yao, Fuguo Zhang, Yaxiao Guo, Heng Wu, Lanlan He, Zhou Liu, Bin Cai, Yu Guo, Calvin J. Brett, Yuanyuan Li, Chinmaya Venugopal Srambickal, Xichuan Yang, Gang Chen, Jerker Widengren, Dianyi Liu, James M. Gardner, Lars Kloo, and Licheng Sun.
  Conformational and Compositional Tuning of Phenanthrocarbazole Based Dopant-Free Hole-Transport Polymers Boosting Performance of Perovskite Solar Cells.
  J. Am. Chem. Soc. 2020, 142, 41, 17681-17692.


• M. Paloncýová , J. Tornmalm, S. Sen, J. Piguet, J. Widengren, and S. Knippenberg.
  Photoisomerization of DiD: Molecular Dynamics Calculations Reveal the Influence of Tail Lengths.
  J. Phys. Chem. C 2020, 124, 10, 5829-5837.


• Yanan Zong, Iva Pruner, Aleksandra Antovic, Apostolos Taxiarchis, Zara Pons Vila, Nida Soutari, Fariborz Mobarrez, Roza Chaireti, Jerker Widengren, Joachim Piguet & Jovan P. Antov.
  Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models.
  Scientific Reports (2020) 10:7871.

2019

• Fuguo Zhang, Zhaoyang Yao, Yaxiao Guo, Yuanyuan Li, Jan Bergstrand, Calvin J. Brett, Bin Cai, Alireza Hajian, Yu Guo, Xichuan Yang, James M Gardner, Jerker Widengren, Stephan V. Roth, Lars Kloo, and Licheng Sun.
  Polymeric, Cost-Effective, Dopant-Free, Hole Transport Materials for Efficient and Stable Perovskite Solar Cells.
  J. Am. Chem. Soc. 2019, 141, 50, 19700-19707.


• Johan Tornmalm, Joachim Piguet, Volodymyr Chmyrov & Jerker Widengren.
  Imaging of intermittent lipid-receptor interactions reflects changes in live cell membranes upon agonist-receptor binding.
  Scientific Reports (2019) 9:18133. https://doi.org/10.1038/s41598-019-54625-w.


• Ingrid Albertsson, Johannes Sjöholm, Josy ter Beek, Nicholas J. Watmough, Jerker Widengren & Pia Ädelroth.
  Functional interactions between nitrite reductase and nitric oxide reductase from Paracoccus denitrificans.
  Scientific Reports (2019) 9:17234. https://doi.org/10.1038/s41598-019-53553-z.


• Johan Tornmalm, Elin Sandberg, Mihailo Rabasovic & Jerker Widengren.
  Local redox conditions in cells imaged via non-fluorescent transient states of NAD(P)H
  Scientific Reports (2019) 9:15070. https://doi.org/10.1038/s41598-019-51526-w.


• Niusha Bagheri, Qingyun Liu, Jan Bergstrand, Rui Pu, Qiuqiang Zhan, Mohammad Hossein Majles Ara, Hans Ågren, Haichun Liu, Jerker Widengren
  Change in the emission saturation and kinetics of upconversion nanoparticles under different light irradiations
  Optical Materials Volume 97, November 2019, p. 109389.


• Jan Bergstrand, Lei Xu, Xinyan Miao, Nailin Li, Ozan Öktem, Bo Franzén, Gert Auer, Marta Lomnytska, Jerker Widengren
  Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells
  Nanoscale 2019 11, p. 10023-10033, DOI: 10.1039/C9NR01967G.


• Jan Bergstrand, Qingyun Liu, Bingru Huang, Xingyun Peng, Christian Würth, Ute Resch-Genger, Qiuqiang Zhan, Jerker Widengren, Hans Ågren and Haichun Liu
  On the decay time of upconversion luminescence
  Nanoscale 2019, 11, 4959-4969, DOI: 10.1039/c8nr10332a.


• Xingyun Peng, Bingru Huang, Rui Pu, Haichun Liu, Tao Zhang, Jerker Widengren, Qiuqiang Zhan and Hans Ågren
  Fast upconversion super-resolution microscopy with 10-μs/pixel dwell times
  Nanoscale 2019, 11, 1563-1559, DOI:10.1039/C8NR08986H.

2018

• Bingru Huang, Jan Bergstrand, Sai Duan, Qiuqiang Zhan, Jerker Widengren, Hans Ågren, and Haichun Liu
  Overtone Vibrational Transition-Induced Lanthanide Excited-State Quenching in Yb3+/Er3+-Doped Upconversion Nanocrystals
  ACS Nano, 2018, 12 (11), pp 10572-10575. DOI: 10.1021/acsnano.8b05095


• Fuguo Zhang, Jiayan Cong, Yuanyuan Li, Jan Bergstrand, Haichun Liu, Bin Cai, Alireza Hajian, Zhaoyang Yao, Linqin Wang, Yan Hao, Xichuan Yang, James M. Gardner, Hans Ågren, Jerker Widengren, Lars Kloo, Licheng Sun
  A facile route to grain morphology controllable perovskite thin films towards highly efficient perovskite solar cells
  Nano Energy Volume 53, November 2018, Pages 405-414.


• Robert Lizatović, Marvin Assent, Arjan Barendregt, Jonathan Dahlin, Anna Bille, Katharina Satzinger, Dagnija Tupina, Albert J. R. Heck, Stefan Wennmalm, Ingemar André
  A Protein-Based Encapsulation System with Calcium-Controlled Cargo Loading and Detachment
  Angew. Chem. Int. Ed. 2018 ,57, 1-6.


• Anuj Pathak, Jan Bergstrand, Vicky Sender, Laura Spelmink, Marie-Stephanie Aschtgen, Sandra Muschiol, Jerker Widengren & Birgitta Henriques-Normark
  Factor H binding proteins protect division septa on encapsulated Streptococcus pneumoniae against complement C3b deposition and amplification
  Nature Communicationsvolume 9, Article number: 3398 (2018).


• Zhixue Du, Jing Yu, Fucai Li, Liyun Deng, Fang Wu, Xiangyi Huang, Jan Bergstrand, Jerker Widengren, Chaoqing Dong, and Jicun Ren.
  In situ monitoring p53 protein and MDM2 protein interaction in single living cells using single molecule fluorescence spectroscopy
  Anal. Chem., 2018, 90 (10), pp 6144-6151, DOI:10.1021/acs.analchem.8b00473.


• Stefan Wennmalm.
  Potentials and pitfalls of inverse fluorescence correlation spectroscopy
  Methods. Volumes 140-141, 1 May 2018, Pages 23-31, DOI:10.1016/j.ymeth.2018.01.005.


• Rudolf Rigler, Jerker Widengren
  Fluorescence-based monitoring of electronic state and ion exchange kinetics with FCS and related techniques: from T-jump measurements to fluorescence fluctuations
  In: European Biophysics Journal, ISSN 0175-7571, E-ISSN 1432-1017, Vol. 47, no 4, p. 479-492.


• Johan Tornmalm, Jerker Widengren.
  Label-free monitoring of ambient oxygenation and redox conditions using the photodynamics of flavin compounds and transient state (TRAST) spectroscopy
  Methods. Volumes 140-141, 1 May 2018, Pages 178-187, DOI:10.1016/j.ymeth.2017.11.013.


• Marta Lomnytska, Rui Pinto, Susanne Becker, Ulla Engström, Sonja Gustafsson, Christina Björklund, Markus Templin, Jan Bergstrand, Lei Xu, Jerker Widengren, Elisabeth Epstein, Bo Franzén and Gert Auer.
  Platelet protein biomarker panel for ovarian cancer diagnosis
  Biomarker Research (2018) 6:2.


• Lei Xu, Laura J. Braun, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström, Annica K. B. Gad
  Nanoscale localization of proteins within focal adhesions indicates discrete functional assemblies with selective force-dependence
  Volume 285, Issue 9, May 2018, Pages 1635-1652.

2017

• Johannes Sjöholm, Jan Bergstrand, Tobias Nilsson, Radek Šachl, Christoph von Ballmoos, Jerker Widengren & Peter Brzezinski.
  The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate
  Scientific Reports 7 2926. DOI:10.1038/s41598-017-02836-4.


• Federico Iovino, Joo-Yeon Engelen-Lee, Matthijs Brouwer, Diederik van de Beek, Arie van der Ende, Merche Valls Seron, Peter Mellroth, Sandra Muschiol, Jan Bergstrand, Jerker Widengren, Birgitta Henriques-Normark.
  pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
  J Exper Med 2017 Archive 5 June 214 (6): 1619.


• Hans Blom and Jerker Widengren.
  Stimulated Emission Depletion Microscopy
  Chem. Rev, 2017, 117 (11), pp 7377-7427. DOI: 10.1021/acs.chemrev.6b00653


• Zhang B, Tornmalm J, Widengren J, Vakifahmetoglu-Norberg H, Norberg E.
  Characterization of the Role of the Malate Dehydrogenases to Tumor Cell Survival.
  Journal of Cancer 2017, 8(11):2088-2096. doi:10.7150/jca.19373.

2016

• Ana M. Villamil Giraldo, Timmy Fyrner, Stefan Wennmalm, Atul N. Parikh, Karin Öllinger, and Thomas Ederth.
  Spontaneous Vesiculation and pH-Induced Disassembly of a Lysosomotropic Detergent: Impacts on Lysosomotropism and Lysosomal Delivery
  Langmuir, 2016, 32 (50), pp 13566-13575 DOI: 10.1021/acs.langmuir.6b03458.


• Vini Nagaraj, Abdulla S. Kazim, Johan Helgeson, Clemens Lewold, Satadal Barik, Pawel Buda, Thomas M. Reinbothe, Stefan Wennmalm, Enming Zhang, Erik Renström.
  Elevated Basal Insulin Secretion in Type 2 Diabetes Caused by Reduced Plasma Membrane Cholesterol
  Molecular Endocrinology Volume: 30 Issue: 10 Pages: 1059-1069, 2016.


• Marcus Cebula, Ilke Simsek Turan, Birgitta Sjödin, Madhuranayaki Thulasingam, Joseph Brock, Volodymyr Chmyrov, Jerker Widengren, Hiroshi Abe, Bengt Mannervik, Jesper Z. Haeggström, Agnes Rinaldo-Matthis, Engin U. Akkaya & Ralf Morgenstern
  Catalytic Conversion of Lipophilic Substrates by Phase constrained Enzymes in the Aqueous or in the Membrane Phase
  Scientific Reports | 6:38316 | DOI: 10.1038/srep38316,2016. Same article in DiVA.


• Heike Hevekerl, Johan Tornmalm, Jerker Widengren
  Fluorescence-based characterization of non-fluorescent transient states of tryptophan - prospects for protein conformation and interaction studies
  Scientific Reports 6:35052 · October 2016 DOI: 10.1038/srep35052., Same article in DiVA.


• Julien R. G. Navarro, Stefan Wennmalm, Jamie Godfrey, Magnus Breitholtz
  Luminescent Nanocellulose Platform: From Controlled Graft Block Copolymerization to Biomarker Sensing
  Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 17, no 3, 1101-1109 p., Same article in DiVA.


• Vini Nagaraj, Abdulla Kazim, Johan Helgeson, Clemens Lewold, Satadal Barik, Pawel Buda, Thomas Reinbothe, Stefan Wennmalm, Enming Zhang, and Erik Renström
  Elevated basal insulin secretion in type 2-diabetes caused by reduced plasma membrane cholesterol
  Mol Endocrinol, (2016) 30 (10): p. 1059-1069, DOI:10.1210/me.2016-1023


• Gábor Mocsár, Julianna Volkó, Daniel Rönnlund, Jerker Widengren, Péter Nagy, János Szöllösi, Katalin Tóth, Carolyn K. Goldman, Sándor Damjanovich, Thomas A. Waldmann, Andrea Bodnár, and György Vámosi
  MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells
  Biophysical Journal Volume 111, Issue 1, p100-112, 2016., Same article in DiVA.


• Lei Xu, Linda Näsvik Öjemyr, Jan Bergstrand, Peter Brzezinski, Jerker Widengren
  Protonation Dynamics on Lipid Nanodiscs: Influence of the Membrane Surface Area and External Buffers
  Protonation Dynamics on Lipid Nanodiscs: Influence of the Membrane Surface Area and External Buffers. Biophysical Journal Volume 110, Issue 9, p1993-2003, 10 May 2016, Same article in DiVA.


• Radek Šachl, Jan Bergstrand, Jerker Widengren and Martin Hof
  Fluorescence correlation spectroscopy diffusion laws in the presence of moving nanodomains
  Journal of Physics D: Applied Physics, Volume 49, Number 11., Same article in DiVA.


• Sunitha Bagawath-Singh, Elina Staaf, Arie Jan Stoppelenburg, Thiemo Spielmann, Taku Kambayashi, Jerker Widengren and Sofia Johansson
  Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells
  Front. Immunol., Vol. 7, 04 February 2016. Same article in DiVA.


• Mihailo D Rabasovic; Evangelos Sisamakis; Stefan Wennmalm; Jerker Widengren
  Label-Free Fluctuation Spectroscopy Based on Coherent Anti-Stokes Raman Scattering from Bulk Water Molecules
  ChemPhysChem ChemPhysChem 2016, 17,p:1025-1033., DOI: 10.1002/cphc.201501129., Same article in DiVA.


• Xu L, Rönnlund D, Aspenström P, Braun LJ, Gad AK, Widengren J
  Resolution, target density and labeling effects in co-localization studies - suppression of false positives by nanoscopy and modified algorithms.
  FEBS Journal 283 (2016) 882-898. doi: 10.1111/febs.13652. Same article in DiVA.

2015

• Martin Larhammar, Kalicharan Patra, Martina Blunder, Lina Emilsson, Christiane Peuckert, Emma Arvidsson, Daniel Rönnlund, Julia Preobraschenski, Carolina Birgner, Christoph Limbach, Jerker Widengren, Hans Blom, Reinhard Jahn, Åsa Wallén-Mackenzie, Klas Kullander
  SLC10A4 Is a Vesicular Amine-Associated Transporter Modulating Dopamine Homeostasi.
  Biological Psychiatry, Volume 77, Issue 6, 15 March 2015, Pages 526-536. Same article in DiVA.


• A. Jamshidi, C. Yuan, V. Chmyrov, J. Widengren, L. Sun, and H. Ågren
  Efficiency Enhanced Colloidal Mn-Doped Type II Core/Shell ZnSe/CdS Quantum Dot Sensitized Hybrid Solar Cells.
  Journal of Nanomaterials Volume 2015 (2015), Article ID 921903, 9 pages. Same article in DiVA.


• Stefan Wennmalm, Volodymyr Chmyrov, Jerker Widengren , and Lars O Tjernberg.
  Highly sensitive FRET-FCS detects amyloid beta-peptide oligomers in solution at physiological concentrations.
  Anal. Chem., 2015, 87 (23), pp 11700-11705 , DOI: 10.1021/acs.analchem.5b02630. Same article in DiVA.


• Hao Xu, Volodymyr Chmyrov, Jerker Widengren, Hjalmar Brismar and Yin Fu.
  Mechanisms of fluorescence decays of colloidal CdSe-CdS/ZnS quantum dots unraveled by time-resolved fluorescence measurement.
  Phys. Chem. Chem. Phys., 2015,17, 27588-27595 DOI: 10.1039/C5CP03109E. Same article in DiVA.


• Heike Hevekerl and Jerker Widengren.
  Determination of molecular stoichiometry without reference samples by analyzing fluorescence blinking with and without excitation synchronization.
  Methods Appl. Fluoresc. 3, 2015, doi:10.1088/2050-6120/3/2/025001. Same article in DiVA.


• Volodymyr Chmyrov , Thiemo Spielmann , Heike Hevekerl , and Jerker Widengren.
  Trans-cis isomerization of lipophilic dyes probes membrane microviscosity in biological membranes and in live cells.
  Anal. Chem., 2015, 87 (11), pp 5690-5697. DOI: 10.1021/acs.analchem.5b00863. Same article in DiVA.

2014

• Denis Doerr, Deborah Sandrin, Stanislav Kalinin, Ralf Kuehnemuth, Sebastian Overmann, Daniela Pfiffi, Klaus Schaper, Claus A.M. Seidel, Thomas J.J. Mueller, Andriy Chmyrov, Jerker Widengren, Brigitte A. Bier.
  Maximizing the Fluorescence Signal and Photostability of Fluorophores by Quenching Dark-States.
  Biophys J Volume 106, Issue 2, Supplement 1, p196a, 28 January 2014. Same article in DiVA.


• Hans Blom, Jerker Widengren.
  STED microscopy -- towards broadened use and scope of applications.
  Current Opinion in Chemical Biology Volume 20 2014, Pages 127-133. DOI: 10.1016/j.cbpa.2014.06.004. Same article in DiVA.


• Heike Hevekerl, Jens Wigenius, Gustav Persson, Olle Inganäs, Jerker Widengren.
  Dark states in ionic oligothiophene bioprobes - evidence from fluorescence correlation spectroscopy and dynamic light scattering.
  The Journal of Physical Chemistry B. 2014 118 (22), pp 5924-5933. DOI:10.1021/jp501324e. , Same article in DiVA.


• Kronqvist N, Otikovs M, Chmyrov V, Chen G, Andersson M, Nordling K, Landreh M, Sarr M, Jörnvall H, Wennmalm S, Widengren J, Meng Q, Rising A, Otzen D, Knight S, Jaudzems K, Johansson J.
  Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation.
  Nature Commun 5, 3254, 2014. , Same article in DiVA.


• Bergstrand J, Rönnlund D, Widengren J, Wennmalm S.
  Scanning Inverse Fluorescence Correlation Spectroscopy.
  Optics Express Vol. 22, No. 11, 13073-13090, 2014. Same article in DiVA.


• Daniel Rönnlund , Lei Xu , Anna Perols , Annica KB Gad , Amelie Eriksson Karlström , Gert Auer , and Jerker Widengren.
  Multicolor Fluorescence Nanoscopy by Photobleaching - Concept, Verification and its Application to Resolve Selective Storage of Proteins in Platelets.
  ACS Nano 2014, 8 (5), pp 4358-4365. Same article in DiVA.


• Jonas Mücksch, Thiemo Spielmann, Evangelos Sisamakis, Jerker Widengren.
  Transient state imaging of live cells using single plane illumination and arbitrary duty cycle excitation pulse trains.
  Journal of Biophotonics Volume 8, Issue 5, pages 392-400, May 2015, DOI:10.1002/jbio.201400015. , Same article in DiVA.


• Xingqi Chen, Chengxi Shi, Samer Yammine, Anita Göndör, Daniel Rönnlund, Alejandro Fernandez-Woodbridge, Noriyuki Sumida, Jerker Widengren, and Rolf Ohlsson.
  Chromatin in situ proximity (ChrISP): Single-cell analysis of chromatin proximities at a high resolution.
  BioTechniques, Vol. 56, No. 3, March 2014, pp. 117-124. Same article in DiVA.


• Annica K B Gad, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström.
  Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy.
  Methods in molecular biology (Clifton, N.J.) 01/2014; 1120:339-57. DOI:10.1007/978-1-62703-791-4_22. Same article in DiVA.


• Ramnath Lakshmanan, Margarita Sanchez-Dominguez, Jose A. Matutes-Aquino, Stefan Wennmalm, and Gunaratna Kuttuva Rajarao.
  Removal of Total Organic Carbon from Sewage Wastewater Using Poly(ethylenimine)-Functionalized Magnetic Nanoparticles.
  Langmuir 2014, 30 (4), pp 1036-1044, dx.doi.org/10.1021/la404076n. , Same article in DiVA.


• Thiemo Spielmann, Lei Xu, Annica K.B. Gad, Sofia Johansson, Jerker Widengren.
  Transient state microscopy probes patterns of altered oxygen consumption in cancer cells.
  FEBS J Volume 281, Issue 5, pages 1317-1332, 2014; DOI: 10.1111/febs.12709. Same article in DiVA.


• Li-Sophie Z. Rathje, Niklas Nordgren, Torbjörn Pettersson, Daniel Rönnlund, Jerker Widengren, Pontus Aspenström, and Annica K. B. Gad.
  Oncogenes induce a vimentin filament collapse mediated by HDAC6 that is linked to cell stiffness.
  PNAS vol. 111 no. 4 1515-1520 2014. , Same article in DiVA.

2013

• Peter D. Zentis, Manuel Frohnapfel, Ville Rantanen, Stefanie Weidtkamp-Peters, Suren Felekyan, Ralf Kuehnemuth, Evangelos Sisamakis, Lei Xu, Anna Perols, Jutta Arden-Jacob, Alexander Zilles, Martina Oberlaender, Britta Fritzsche, Per-Åke Nygren, Amelie Eriksson Karlström, Sampsa Hautaniemi, Karl-Heinz Drexhage, Gert Auer, Jens K. Habermann, Jerker Widengren, Claus A. M. Seidel,
  Cancer Diagnostics by Multiparameter Fluorescence Image Spectroscopy: A Bioinformatic Classifier Trained on Cultured Immunostained Cells
  Biophys J Volume 104, Issue 2, Supplement 1, p342a, 29 January 2013 , Same article in DiVA.


• Hans Blom, Daniel Rönnlund, Lena Scott, Linda Westin, Jerker Widengren, Anita Aperia, Hjalmar Brismar.
  Spatial Distribution of DARPP-32 in Dendritic Spines.
  PloS ONE 8(9) 2013: e75155. doi:10.1371/journal.pone.0075155. Same article in DiVA.


• Daniel Rönnlund, Annica K. B. Gad, Hans Blom, Pontus Aspenström, Jerker Widengren.
  Spatial Organization of Proteins in Metastasizing Cells.
  Cytometry Part A 2013, Volume 83, Issue 9, pages 855-865, DOI: 10.1002/cyto.a.22304. , Same article in DiVA.


• Jerker Widengren.
  Studying Ion Exchange in Solution and at Biological Membranes by FCS.
  Methods in Enzymology, Volume 519, 231-252. Same article in DiVA.


• Paul J. Rothwell, William J. Allen, Evangelos Sisamakis, Stanislav Kalinin, Suren Felekyan, Jerker Widengren, Gabriel Waksman and Claus A. M. Seidel.
  dNTP-dependent Conformational Transitions in the Fingers Subdomain of Klentaq1 DNA Polymerase INSIGHTS INTO THE ROLE OF THE "NUCLEOTIDE-BINDING" STATE.
  The Journal of Biological Chemistry, 288 (19), 13575-13591., doi: 10.1074/jbc.M112.432690. Same article in DiVA.

2012

• Stefan Wennmalm and Jerker Widengren.
  Interferometry and Fluorescence Detection for Simultaneous Analysis of Labeled and Unlabeled Nanoparticles in Solution.
  J. Am. Chem. Soc., 2012, 134 (48), pp 19516-19519, DOI: 10.1021/ja308213q. , Same article in DiVA.


• Daniel Rönnlund, Yang Yang, Hans Blom, Gert Auer, Jerker Widengren.
  Fluorescence Nanoscopy of Platelets Resolves Platelet-State Specific Storage, Release and Uptake of Proteins, Opening for Future Diagnostic Applications.
  Adv Healthcare Mat 2012, online: 7 Sep, DOI: 10.1002/adhm.201200172. , Same article in DiVA.


• Michele Cristovao, Evangelos Sisamakis, Manju M. Hingorani, Andreas D. Marx, Caroline P. Jung, Paul J. Rothwel,, Claus A. M. Seidel, and Peter Friedhoff.
  Single-molecule multiparameter fluorescence spectroscopy reveals directional MutS binding to mismatched bases in DNA.
  Nucl. Acids Res. (2012), 40 (12):5448-5464, doi:10.1093/nar/gks138. Same article in DiVA.


• Annica K. B. Gad, Daniel Rönnlund, Alexander Spaar, Andrii A. Savchenko, Gabor Petranyi, Hans Blom, Laszlo Szekely, Jerker Widengren and Pontus Aspenström.
  Rho GTPases link cellular contractile force to the density and distribution of nanoscale adhesions.
  FASEB J 2012, vol. 26, no. 6 2374-2382. , Same article in DiVA.


• Peter Mellroth, Robert Daniels, Alice Eberhardt, Daniel Rönnlund, Hans Blom, Jerker Widengren, Staffan Normark and Birgitta Henriques-Normark.
  LytA, the major autolysin of Streptococcus pneumoniae, requires access to the nascent peptidoglycan.
  J Biol Chem 2012 Vol. 287, 14:11018-11029. Same article in DiVA.


• Karl Skoog, Bill Söderström, Jerker Widengren, Gunnar von Heijne and Daniel O. Daley.
  Sequential Closure of the Cytoplasm and Then the Periplasm during Cell Division in Escherichia coli.
  J. Bacteriol. February 2012 vol. 194 no. 3:584-586. Same article in DiVA.


• Tor Sandén, Romain Wyss, Christian Santschi, Ghérici Hassaïne, Cédric Deluz, Olivier J.F. Martin, Stefan Wennmalm, and Horst Vogel.
  A Zeptoliter Volume Meter for Analysis of Single Protein Molecules.
  Nano Lett 2012, 12(1), 370-375. Same article in DiVA.


• Hans Blom, Daniel Rönnlund, Lena Scott, Zuzana Spicarova, Ville Rantanen, Jerker Widengren, Anita Aperia and Hjalmar Brismar.
  Nearest neighbor analysis of dopamine D1 receptors and Na⁺-K⁺-ATPases in dendritic spines dissected by STED microscopy.
  Microscopy Research and Technique 2012 Vol. 75, Issue 2, 220-228. , Same article in DiVA.

2011

• Heike Hevekerl, Thiemo Spielmann, Andriy Chmyrov, and Jerker Widengren.
  Förster Resonance Energy Transfer beyond 10 nm: Exploiting the Triplet State Kinetics of Organic Fluorophores.
  J Phys Chem B, 2011, 115 (45), pp 13360-13370. Same article in DiVA.


• Johan Strömqvist, Sofia Johansson, Lei Xu, Yu Ohsugi, Katja Andersson, Hideki Muto, Masataka Kinjo, Petter Höglund and Jerker Widengren.
  A Modified FCCS Procedure Applied to Ly49A-MHC Class I cis-Interaction Studies in Cell Membranes.
  Biophysical Journal 5, 1257-1269, 2011. Same article in DiVA.


• Jens Wigenius, Gustav Persson, Jerker Widengren, Olle Inganäs.
  Interactions Between a Luminescent Conjugated Oligoelectrolyte and Insulin During Early Phases of Amyloid Formation.
  Macromolecular Bioscience Vol. 11, no. 8, 1120-1127, 2011. Same article in DiVA.


• Stefan Wennmalm, Jerker Widengren.
  Inverse-fluorescence correlation spectroscopy: more information and less labeling.
  Frontiers in Bioscience S3, 385-392, January 1, 2011. Same article in DiVA.


• Hans Blom, Daniel Ronnlund, Lena Scott, Zuzana Spicarova, Jerker Widengren, Alexander Bondar, Anita Aperia and Hjalmar Brismar.
  Spatial distribution of Na⁺,K⁺-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy.
  BMC Neuroscience 2011, 12:16/1471-2202-12-16. Same article in DiVA.

2010

• Johan Strömqvist, Andriy Chmyrov, Sofia Johansson, August Andersson, Lena Mäler and Jerker Widengren.
  Quenching of Triplet State Fluorophores for Studying Diffusion-Mediated Reactions in Lipid Membranes.
  Biophys J. 2010, Volume 99, Issue 11, 3821-3830. , Same article in DiVA.


• Andriy Chmyrov, Tor Sandén and Jerker Widengren.
  Recovery of Photoinduced Reversible Dark States Utilized for Molecular Diffusion Measurements.
  Anal. Chem., 2010, 82 (24), pp 9998-10005. Same article in DiVA.


• Andriy Chmyrov, Tor Sandén and Jerker Widengren.
  Iodide as a Fluorescence Quencher and Promoter--Mechanisms and Possible Implications.
  J. Phys. Chem. B, 2010, 114 (34), pp 11282-11291. Same article in DiVA.


• Evangelos Sisamakis, Alessandro Valeri, Stanislav Kalinin, Paul J. Rothwell and Claus A.M. Seidel.
  Chapter 18 - Accurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection.
  In: Methods in Enzymology Volume 475, 2010, Pages 455-514. Same article in DiVA.


• Stefan Wennmalm and Jerker Widengren.
  Inverse-Fluorescence Cross-Correlation Spectroscopy.
  Anal. Chem. 2010, 82, 5646-5651. Same article in DiVA.


• Stanislav Kalinin, Evangelos Sisamakis, Steven W. Magennis, Suren Felekyan and Claus A. M. Seidel.
  On the Origin of Broadening of Single-Molecule FRET Efficiency Distributions beyond Shot Noise Limits.
  J. Phys. Chem. B, 2010, 114 (18), 6197-6206. , Same article in DiVA.


• Widengren J.
  Fluorescence-based transient state monitoring for biomolecular spectroscopy and imaging.
  Journal of the Royal Society Interface August 6, 2010 7:1135-1144. , Same article in DiVA.


• Tor Sandén, Lina Salomonssona, Peter Brzezinski, and Jerker Widengren.
  Surface-coupled proton exchange of a membrane-bound proton acceptor.
  Proc. Natl. Acad. Sci. USA 2010 vol. 107(9), 4129-4134. , Same article in DiVA.


• Johan Strömqvist, Karl Skoog, Daniel O. Daley, Jerker Widengren and Gunnar von Heijne.
  Estimating Z-ring radius and contraction in dividing Escherichia coli.
  Molecular Microbiology 2010, 76(1), 151-158. , Same article in DiVA.


• Thiemo Spielmann, Hans Blom, Matthias Geissbuehler, Theo Lasser and Jerker Widengren.
  Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy.
  J. Phys. Chem. B 2010, 114 (11), 4035-4046. , Same article in DiVA.


• Hans Blom, Kai Hassler, Andriy Chmyrov and Jerker Widengren.
  Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy.
  Int. J. Mol. Sci. 2010, 11(2), 386-406. Same article in DiVA.

2009

• Widengren J.
  Fluorescence flicker as a read-out in FCS -principles, applications and further developments.
  in Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Verlag 2009, Eds. Gräslund, Rigler, Widengren. , Same article in DiVA.


• Hongzhen Lin, Yuxi Tian, Karolina Zapadka, Gustav Persson, Daniel Thomsson, Oleg Mirzov, Per-Olof Larsson, Jerker Widengren, and Ivan G. Scheblykin.
  Fate of Excitations in Conjugated Polymers: Single-Molecule Spectroscopy Reveals Nonemissive "Dark" Regions in MEH-PPV Individual Chains.
  Nano letters, 2009, 9 (12), pp 4456-4461. Same article in DiVA.


• Stefan Wennmalm, Per Thyberg, Lei Xu and Jerker Widengren.
  Inverse-Fluorescence Correlation Spectroscopy.
  Anal. Chem., 2009, 81 (22), pp 9209-9215. Same article in DiVA.


• Hans Blom and Gunnar Björk.
  Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy.
  Applied Optics, Vol. 48, Issue 31, pp. 6050-6058. Same article in DiVA.


• Gustav Persson, Tor Sandén, AnnSofi Sandberg and Jerker Widengren.
  Fluorescence cross-correlation spectroscopy of a pH-sensitive ratiometric dye for molecular proton exchange studies.
  Phys Chem Chem Phys, 2009, 11, 4410-4418. Same article in DiVA.


• Gustav Persson, Per Thyberg, Tor Sandén and Jerker Widengren.
  Modulation Filtering Enables Removal of Spikes in Fluorescence Correlation Spectroscopy Measurements without Affecting the Temporal Information.
  J Phys Chem B, 2009, 113 (25), 8752-7. Same article in DiVA.


• Hans Blom, Andriy Chmyrov, Kai Hassler, Lloyd M. Davis and Jerker Widengren.
  Triplet-State Investigations of Fluorescent Dyes at Dielectric Interfaces Using Total Internal Reflection Fluorescence Correlation Spectroscopy.
  J. Phys. Chem. A, 2009, 113 (19), 5554-66. Same article in DiVA.


• Gustav Persson, Tor Sandén, Per Thyberg, and Jerker Widengren.
  Modulated or alternating excitation in fluorescence correlation spectroscopy.
  Proc. SPIE, Vol. 7185, 718509 (2009); doi:10.1117/12.808912. , Same article in DiVA.


• Tor Sandén, Gustav Persson, Jerker Widengren.
  Transient state microscopy: a new tool for biomolecular imaging (Proceedings Paper).
  Proceedings Vol. 7183. Multiphoton Microscopy in the Biomedical Sciences IX, Ammasi Periasamy; Peter T. C. So, Editors, 71832R. , Same article in DiVA.


• Öjemyr L, Sandén T, Widengren J, and Brzezinski P.
  Lateral Proton Transfer between the Membrane and a Membrane Protein.
  Biochemistry, 2009, 48 (10), 2173-2179. Same article in DiVA.

2008

• Chmyrov A, Arden-Jacob J, Zilles A, Drexhage KH and Widengren J.
  Characterization of new fluorescent labels for ultra-high resolution microscopy.
  Photochem. Photobiol. Sci., 2008, 7, 1378-1385. , Same article in DiVA.


• Sandén T, Persson G, and Widengren J.
  Transient State Imaging for Microenvironmental Monitoring by Laser Scanning Microscopy.
  Anal Chem, 2008, 80 (24), 9589-9596. , Same article in DiVA.


• Persson G, Thyberg P, Widengren J.
  Modulated Fluorescence Correlation Spectroscopy with Complete Time Range Information.
  Biophysical Journal vol. 94, 977-85 2008. Same article in DiVA.


• Sonesson A, Blom H, Hassler K, Elofsson U M, Callisen T H, Widengren J, Brismar H.
  Protein-surfactant interactions at hydrophobic interfaces studied with total internal reflection fluorescence correlation spectroscopy (TIR-FCS).
  Journal of Colloid and Interfaces Science 317, 449-57, 2008. Same article in DiVA.

2007

• Sandén T, Persson G, Thyberg P, Blom H, Widengren J.
  Monitoring kinetics of highly environment-sensitive states of fluorescent molecules by modulated excitation and time-averaged fluorescence intensity recording.
  Anal. Chem. 79(9), 3330-3341, 2007. Same article in DiVA.


• Widengren J, Chmyrov A, Eggeling C, Löfdahl PÅ, Seidel CAM.
  Strategies to improve photostability in ultrasensitive fluorescence spectroscopy.
  J. Phys. Chem. A 111(3), 429-440, 2007. Same article in DiVA.


• Hassler K, Rigler P, Blom H, Rigler R, Widengren J, Lasser T.
  Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy.
  Optics Express, 15(9), 5366-5375, 2007. Same article in DiVA.

2006

• Widengren J, Kudryavtsev V, Gerken M, Berger S, Seidel CAM.
  Single molecule detection and identification of multiple species by multiparameter fluorescence detection.
  Analytical Chemistry 78(6); 2039-2050, 2006. Same article in DiVA.


• Eggeling C, Widengren J, Brand L, Schaffer J, Felekyan S, Seidel CAM.
  Analysis of Photobleaching in Single-Molecule Multicolor Excitation and Förster Resonance Energy Transfer Measurements.
  J. Phys. Chem A 110(9); 2979-2995, 2006, Same article in DiVA.


• Leutenegger M, Blom H, Widengren J, Eggeling C, Gösch M, Leitgeb RA, Lasser T.
  Dual-color total internal reflection fluorescence cross-correlation spectroscopy.
  J Biomed Optics, 11(4), 2006. Same article in DiVA.


• Brändén M, Sandén T, Brzezinski P, Widengren J.
  Localised proton microcircuits at the biological membrane-water interface.
  Proc. Natl. Acad. Sci. U.S.A. 103(52), 19766-19770, 2006. Same article in DiVA.


• Blom H, Kastrup L, Eggeling C.
  Fluorescence Fluctuation Spectroscopy in Reduced Detection Volumes.
  Current Pharmaceutical Biotechnology. 7(1), 51-66, 2006. Same article in DiVA.

2005

• Widengren J, Thyberg P.
  FCS cell surface measurements - photophysical limitations and consequences on molecular ensembles with heterogenic mobilities.
  Cytometry part A, Vol. 68, 2: 101-112, 2005. Same article in DiVA.

2004

• Fridberger A, Widengren J, Boutet de Monvel J.
  Measuring hearing organ vibration patterns with confocal microscopy and optical flow.
  Biophys. J. 86 (1): 535-543, 2004. Same article in DiVA.


• Pramanik A, Widengren J.
  Fluorescence Correlation Spectroscopy (FCS).
  in Encyclopedia of Molecular Cell Biology and Molecular Medicine, Wiley-VCH Verlag 2004, p. 461-500, ed. Meyers , Same article in DiVA.

2003

• Margittai M, Widengren J, Schweinberger E, Schroder GF, Felekyan S, Haustein E, Konig M, Fasshauer D, Grubmuller H, Jahn R, Seidel CAM.
  Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1.
  Proc. Natl. Acad. Sci. U.S.A. 100 (26): 15516-15521, 2003. , Same article in DiVA